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fighton (25)in #eos • 7 years ago
RE: Why you want to support this Project
I do CRISPR every day and have made dozens of genetically modified cell lines. I can easily do this, but your article is overly simplified. Having said that, I haven't done any…
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fighton (25)in #health • 7 years ago
RE: Are We Playing God? CRISPR Gene Editing
Antibiotics work on bacteria, so viruses are not effected by antibiotics. Do not take antibiotics for a viral infection. Now that that is out of the way, I like the idea of…
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fighton (25)in #health • 7 years ago
RE: Are We Playing God? CRISPR Gene Editing
This paper has since been retracted. Their data was poorly obtained and incorrectly analyzed.
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fighton (25)in #science • 7 years ago
RE: Let's Splice! (My Research, Vol. 3)
@suesa why not flox the gene of interest you want to knockout, and do tissue specific Cre expression by throwing in an inducible Cre driven by a neuron specific promoter? I guess…
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fighton (25)in #science • 7 years ago
RE: Gene Editing - Cre/loxp (My Research, Vol. 2)
Cool article, but the Cre/Loxp system will (most likely) never be used in a clinical setting for humans, at least not in the United States or anywhere with a decent regulatory…
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fighton (25)in #science • 7 years ago
RE: Gene Editing - Cre/loxp (My Research, Vol. 2)
@schay and @suesa send me any CRISPR/Cas9 questions you have. I use it every day to make new human stem cell lines.
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fighton (25)in #science • 7 years ago
RE: Gene Editing - Cre/loxp (My Research, Vol. 2)
Gene editing is a bit anti-climactic and is mostly imagination and your own knowledge. Example: Mix a drop of this colorless liquid with a drop of that colorless liquid. Put…
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fighton (25)in #biology • 7 years ago
RE: Cutting, Pasting, Cloning, and GMOs
I use Gibson assembly when I generate most plasmids, but sometimes you can't. But you'd be surprised how few people use Gibson assembly or even understand it. It's very useful…
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fighton (25)in #biology • 7 years ago
RE: Sequencing DNA using Gel Electrophoresis
Good, nicely explained article :) Easy to follow
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fighton (25)in #science • 7 years ago
RE: Fascinating genetics #Cloning vectors, The plasmid.
I think there is still too much controversy going around about who discovered CRISPR/Cas9 versus who figured out its best use. Additionally, the Nobel prize isn't awarded…
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fighton (25)in #steemstem • 7 years ago
RE: Activate genes with light! - Optogenetic control of intracellular signalling
I used channelrhodpsin when I figured out a novel way to reprogram/transdifferentiate human skin cells directly into skeletal muscle. I would shine blue light on the cells…
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fighton (25)in #steemstem • 7 years ago
RE: Gene Drive and the Engineering of Extinction – Part I
Hi @kilbride and @carlgbush. I perform CRISPR/Cas9 gene editing in human pluripotent stem cells, so I am familiar with these topics. Yes, self damage and apoptosis (termination…
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fighton (25)in #biology • 7 years ago
RE: The Most Beautiful Experiment In Biology
No need to synthesize the plasmid, you can just grow that plasmid in bacteria that have the isotope present in the media. But even then, the results would just say something…
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fighton (25)in #biology • 7 years ago
RE: The Most Beautiful Experiment In Biology
Cool idea, but that would require a lot of money and is not technologically feasible currently, given that synthesized DNA can only be synthesized up to a couple thousand base…
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