A pH-regulated nanopore having smaller surface charge density but remarkably higher performance of nanofluidic salinity gradient power (NSGP) is demonstrated for the first time. The counterintuitive surface-charge-dependent NSGP behavior is also shown resulting from the pH-dependent ion concentration polarization effect, which yields the degradation in effective concentration ratio across a nanopore.P5KRRWu38rz3V49CKdziPwigbDtLq5Lmr8s2Pyjyr