Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

in Informationwar3 years ago

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988269/

In the present case of 2019-nCoV, virus isolates or samples from infected patients have so far not become available to the international public health community. We report here on the establishment and validation of a diagnostic workflow for 2019-nCoV screening and specific confirmation, designed in absence of available virus isolates or original patient specimens. Design and validation were enabled by the close genetic relatedness to the 2003 SARS-CoV, and aided by the use of synthetic nucleic acid technology.