Practical Microbiology: The Indicative Smell

in STEMGeeks5 years ago

         Well, the thing about working in an understaffed situation is that new hires are coming. And some of them have. Now, the training part could take a while. The fun part about training someone is that you get to know your new coworker. At the same time, share experiences relevant to the job.

         One of the major things lab technologists do is identifying pathogens. Outside of biochemical tests, etc., we use different clues to work towards a conclusion. One of such methods is the smell of the organisms. Of course, there is safety precautions to taking whiff, so that's a given.

         With the internet, it's often easy to find resources to use as aid for training. Here's an example of a chart I found to help with training.


Source

         For the most part, I agree with the chart. There are some of them I would disagree with based on my experience.

For example:

Citrobacter - more of a brussel sprout smell. This is more true on chrome agar than not.

Corynebacterium - I have never encountered fruity smell on any plate.

Haemophilus - it has a smell, but doesn't seem to resemble wet far as the website suggests.

Nocardia - more of a rain smell. Instead of musty, it's actually a lot fresher.

Pseudomonas - can also smell like tortilla chips depending on the variant.

         An off chart example would be Staphylococcus saprophyticus. It shares the dirty sneaker/socks smell like S. aureus. On the chrome agar, the smell can be a differentiating point from other Staph species. Often, the smell is what sets S. saprophyticus and S. simulans apart on the chrome agar. Unless, of course, you choose to spend time using the MALDI-TOF to identify them for sure.

Why the smell?

         Sometimes, cultures can be messy. As in, several organisms can be present. Your sight may not always pick up all the different colonies that exist on the plate. Being able to pick out a distinct smell could help you find an organism you didn't see.

         On the efficiency side, you could save yourself some time and effort. This is especially true for some cultures where the susceptibilities are the focus. This also includes more preliminary information for the clinicians.

         Let's take the viridans group. Noticing that buttery smell helps you declare that you don't have S. pneumoniae. This is especially important in a respiratory culture.

         With Nocardia species, sometimes the smell come before you notice distinct colonies. This is one pathogen you want to pick out asap when dealing with blood cultures.

         I could go on and on, but the point is, smell can be a useful tool in microbiology. With the new dress code due to the pandemic, one should feel a bit safer taking a whiff. Everyone is pretty much in biohazard level 2 attire now.

         Anyways, it's another night at the lab.

Sort:  

The other weekend when I was talking to that lady who works in the crime lab she was saying that Cocaine has a very distinct odor. If I remember right she said it was very vinegary. She said that when they have a potential cocaine sample come in they always fight over it, especially when one of them has a cold because it clears your sinuses right out.

 5 years ago  

Today I learned...

Niceee, i study microbiology, and microbiology advanced, i remember the test im gram! Loved the colors in the bacterys !discovery 15

 5 years ago  

Nice to see a fellow microbiologist. I've only encountered a few with that discipline on Hive.

I guess I would not be suitable for that type of a job, because my sniffer does not work well with a lot of smells, things really have to smell for me to smell them.

Burnt chocolate I have never smelled before, maybe bad cooks have, but I have never smelled burnt chocolate, and acrid, what kind of smell is that? and pungent (indole)? When in school do they make you smell those things?

 5 years ago  

I'm of the personal opinion that I have a bad sense of smell. If I could do it, you could too.

A lot of the smells require you to experience it in order to relate. I don't recall them forcing us to smell everything back in school. But, the smells are super helpful.

The burnt chocolate one is interesting because I have heard some folks say it smells disgusting. To me, it's just a light cocoa smell. So, everyone's mileage varies.


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 5 years ago  Reveal Comment
 5 years ago  

I got 13 people following my flags.

I didn't think you'd be dumb enough to rattle the cage. Expect more people come flagging you.

 5 years ago  

Then, don't do dumb shit.

Don't downvote strangers on the internet then, moron. You came downvoting me expecting anything less in return?

Stay on those blacklists.

Dear @steemflagrewards

I've noticed that you've been downvoting our content lately and I was wondering if there is any reason for your actions?

Did I do anything to upset you? Just curious.


Yours, @crypto.piotr @project.hope founder

 5 years ago (edited) 

I don't need someone who pretends to be a dozen people to say that ironically.