As promised in my earlier post about sterilizing tomato seed below I show how I prepare media for tissue culture purposes. In this case I am germinating tomato seed in an effort to end up with sterile plant material to work with in vitro. The media is called standard M&S mixtrophic mixture or MSO. M stands for Murashige and S stands for Skoog. A number behind the letters MS is used to indicate the sucrose concentration of the medium. For example, MS0 contains no sucrose and MS20 contains 20 g/l sucrose.
MSO was invented by plant scientists Toshio Murashige and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator.
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators
Wow.great video for me.cause i am a student of science.and tissue culture media is needed to complete my practical class.tnx
Pls give an upvote there https://steemit.com/science/@masudrana/if-you-want-to-increase-the-size-of-your-iot-keep-thinking
Cool post on IOT! Where are you from? I'm glad to hear this post will help you in your studies. Let me know if any other tissue culture or plant science related topics would be of interest to you.