In my opinion, the very minute they ate of the tree, they died! Gen 3:7 reports that the eyes of both were naked and they knew that they were naked...
no read it this way the eyes of both were naked and they knew that they were died...this is because death is the end of the life of a person or organism; before this time, Adam and his wife were living the God life of Glory. there is no replacement for glory, it is either glory (life) or shame (death). the eating of the fruit created a permanent ending of vital processes in the supernatural. they began to do things that they had no knowledge of, for they first time, they heard the voice of God "walking"; they understood nakedness - voidness and emptiness without being taught; they adopted complaints (shifting blames) without be taught instead of accepting responsibilty. Before his death (the fruit), Adam was hundred percent a spirit man and his death made him physical.
fast forward, when the Lord God decided to redeem the world back to Himself, the process had to be taken, again. Having partaking of the tree of through Adam's (first Adam)disobedience we also had to partake of the tree of Life through Jesus (last Adam) obedience (Romans 5:19).
Adam's death was by disobedience that pushed man away from God BUT Jesus' death was by Obedience that brought man back to God. Death in this cases opened the chapter to another life; why for the first Adam it was to eternal damnation, for the last Adam it was to eternal Life. And mind you, eternal life is not a 'far-fetched, when you die you receive it thing' it is a life in Christ. Just as after Adam's death by eating the fruit men lived in the chapter opened by that act, when you accept Jesus as your Lord and Saviour, you begin to live eternal life in the world. Cause His death made it possible that as He is in Heaven, so are we on earth!
this is my opinion