Hello all steemian friends, this time I will share some of my experience with other science teachers when attending training on improving the ability of teachers in learning science. And a lot of that we study here but on this occasion I will share my experience on technique breeding and observe the morphology of bacteria.
Bacillus
Bacillus sp is a rod-shaped bacteria, and belongs to gram-positive bacteria, can generally grow on oxygen-containing medium, the bacterium is also known as aerobic sporefomers. But most members of the genus Bacillus sp. can form an intracellularly formed endospores that are in response to adverse environmental conditions, therefore the genus member Bacillus sp. has a high tolerance for changing environmental conditions.
Bacillus is a genus of gram-positive, rod-shaped bacteria and a member of the phylum Firmicutes. Bacillus species can be obligate aerobes (oxygen reliant), or facultative anaerobes (having the ability to be aerobic or anaerobic). They will test positive for the enzyme catalase when there has been oxygen used or present. [1]
We know that Bacteria have distinctive morphological features, structures and traits. The scientists classify that bacteria are microorganisms that have microscopic size. Bacteria are also colorless or transparent and contrast with water. So to observe bacteria in life condition is very difficult. So to overcome this then developed a bacterial cell staining technique. This is one of the ways in microbiological research in order to facilitate the identification of the bacteria.
Me and the other science teachers before doing the experiments first hear the briefing from the laboratory instructor on the steps to be taken.
What we did here!!!
First we do is create a medium with a mixture of nutrients to grow microbes. In order for cultured microorganisms to grow well in the medium, it should be noted that things (1) media must contain nutrients that are easy to use by microorganisms (2) the media must have an appropriate osmose pressure and pH (3) inhibiting bacterial growth and most importantly (4) must be sterile.
After completion of such media, individual microbial cells will multiply rapidly, so that within approximately 24 hours the formation of a cell mass can be observed and this is called a colony.
We and science teachers have been training for a week so have plenty of opportunities to gain a range of science knowledge.
After we had bacteria in the medium, the next day we were looking at the microscope to see the morphological shape of the bacteria. Where the results we see in the form of their colonies Bacteria (bacillus/singgle) according to their names, are often found in a state of solitude, Bacteria diplobacilli (paired) are bacteria that are found often in pairs. And there is also Bacteria streptobacilli (chains) is a bacterial colony that forms a chain.
Conclusion
From the experience our experiment we have done a lot of new information that we got. We already know and understand the procedure of gram staining and bacterial grouping. Bacillus is a gram-positive bacteria, because in this bacterium contains many peptidogligans so easily bind to the purple crystals.
So that at the last staining of bacteria into blue or purple. Although this research has been done a lot but to understand more the latest information is of course very important for us all. So that our knowledge is increasing and we can apply for learners for a teacher.