The gene of ghrelin (ghrl) (3p26-p25) is responsible for the synthesis of a hormone involved in the stimulation of appetite and storage of fat in adipose tissue (obestatin), which is why it is associated with obesity, multifactorial degenerative chronic disease, capable of increasing the risk of chronic diseases such as DM2 and cardiovascular diseases. The search for targets for gene therapy remains a challenge, and it has been described that the frequency of single nucleotide polymorphisms (SNPs) in genes susceptible to DM2 as ghrl may compromise the effectiveness of gene editing technologies based on RNA-guides of interference (sgRNAs) in CRISPR/Cas systems. Therefore, we sought to determine the frequency of SNPs in the gene candidate for DM2 ghrl in silico. For the Screening of SNPs the NCBI/GenBank and the databases were used: 1000Genomes Project in phase-3, GnomAD, TOPMED, ALSPAC and TWINSUK, gathering the genotype of >172 700 individuals worldwide. A total of 711 SNPs were found, of which 80% statistically presents the lowest relative allelic frequency (≤0.1%), 13% exhibited a relative frequency of between 10-20% and 7% showed a relative frequency of SNPs [C>T] ≥35%. All the observed allelic frequencies are very high in terms of probabilistic occurrence. Additionally, all SNPs were located in introns. These findings should be taken into account because although introns do not encode proteins, SNPs in introns can affect gene expression, and although most of the changes are silent and have no functional repercussions, the large number of SNPs found suggest that the specificity of genetic therapies based on the design of sgRNAs may be affected because of the evident genetic variability to which they are exposed the targets associated with DM2, deserving more studies to fine-tune the effectiveness of this technology with the interest of being able to promote its application at a clinical level.
That's exquisite and incredible!!
Hello Igonzalezpaz. What I would do right now is not post. This is advice and I am trying to help guide you. Read some posts and comment on them properly and grow your following before you post. It may take a month but you won't get many rewards for the effort you put in.
Once you have followers you can then post. It takes time but you will grow and this is the quickest way.
Thank you very much for the suggestion, I'll take it, I'm actually new to this, but I appreciate your advice ..
I saw that and don't want you to fall into the trap that 99% make and waste a month. Start slowly and find one or two new people a day and slowly build your account. Within a month you will have 20 to 30 genuine followers. It takes time just build your foundations first.
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