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RE: Microinjections - The Creation of Transgenic Mice (My Research, Vol. 6)

in #science7 years ago

Until 12-14 hours after mating, the mouse vagina is "blocked" by a so-called "plug". It's dried up liquid that came with the sperm. So, you put the females in a cage with the males, and 11-12 hours later, you check for plugs. Females with a plug can be uses 2 days later for the embryo transfer.

We usually inject the eggs at the blastocyst stage or slighty before, about 3 days after the microinjection of the components.

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I didn't know about the plug thing , i do understand now ! anyway looking forward to see your newborns mice :D