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RE: Microinjections - The Creation of Transgenic Mice (My Research, Vol. 6)

in #science7 years ago

Yes, you're correct, just injecting mRNA isn't enough. In fact, in the step described I only tested my mRNA. It's mRNA coding for Cre recombinase, which is part of my gene editing "set". I needed to know if it's functional, before injecting it together with all other components into the zygotes I wanted to actually place in mice.

C. elegans and D. melanogaster are certainly a lot faster, I agree :P