Much of the time this works great! Just a few head's up in case you run into issues
- some Ni resins can non-selectively oxidize your proteins and
- if you're expressing in E. coli, slyD can contaminate nickel based IMAC. IF that's an issue and IF it's occuring, switing to a cobalt based resin can help.
Wow!!! Thanks for the amazing comments!!! I never know about that!!!
I didn't know cobalt also works!!!!! )0(
Yep, not an endorsement of a particular product, but this is the type of stuff I was talking about.