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[mcw]

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Comments

[effofex]

Much of the time this works great! Just a few head's up in case you run into issues

• some Ni resins can non-selectively oxidize your proteins and
• if you're expressing in E. coli, slyD can contaminate nickel based IMAC. IF that's an issue and IF it's occuring, switing to a cobalt based resin can help.

[mcw]

Wow!!! Thanks for the amazing comments!!! I never know about that!!!
I didn't know cobalt also works!!!!! )0(

[effofex]

Yep, not an endorsement of a particular product, but this is the type of stuff I was talking about.

[nostalgic1212]

细菌在我这样的外行人眼里简直就是“害虫”(´▽｀)mei 想到在实验室里，还能作为小帮手呢

[mcw]

哈哈
對啊，因爲細菌生長得快，所以訓一晚翌日就有成果了 XDDD
其實我們的腸道有很多益生菌的啊！！

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